Well said Melissa. I have done both ways.
I can see several generations in that colony. An easy to to see if they are mature enough is to give each a little tug, to see if they are already rooted. If they are you can remove the now, this is the time of year that they grow the fastest and will make the change quickly. In a short while all of the will be sending out new offsets.
I would give the older ones enough space that their new offsets.
Tim does this and all of his colonies are beautiful.