chalyse said:Do I understand correctly that the need for chemical pre-treament is just for 1) Colchicine to make chromosomes easier to see, and 2) HCI to soften the cells so that they can be squashed more easily into a single layer without bursting? If a household alternate (say, vinegar) were possible to substitute for the HCI, softening the cells over days instead of minutes, and we took out the Colchicine step altogether during pre-treatment ... would the chromosomes still be too difficult to see, even at higher magnification?
The current source of my continuing curiosity, where Colchicine is not mentioned: http://people.bridgewater.edu/...
and a "yahoo" answer about HCI substitutes that piques my curiosity: "vinegar is a weak acid and is used in high school chem labs all the time because it is a lot safer than using stronger acids"