Viewing post #542534 by admmad

You are viewing a single post made by admmad in the thread called Converting Tetraploids to Diploids - Is There a Process?.
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Jan 21, 2014 7:21 AM CST
Name: Maurice
Grey Highlands, Ontario (Zone 5a)
To be able to see and count chromosomes requires that a procedure is followed using various chemicals as well as a reasonable microscope. I would guess you could purchase one on e-bay but it would probably need to be able to magnify 400 to 500x and probably cost around $500-$600 or much more.

Colchicine is often used (or at least it was back when I made chromosome squashes) to make chromosomes shorter and thicker and easier to count. Hydrochloric acid, heated for a few minutes is used to make the plant tissue softer and easier to squash so that the chromosomes can be spread apart so that they are easier to count. Chemical stains are used to make the chromosomes visible. Some of these chemicals may have been replaced with other more modern chemicals but the basic process has not changed much.

The root tips are the tissue usually used to count chromosomes although the very early stages of pollen production can also be used. However, those very early stages occur in buds that are very small and it would probably require another different (dissecting) microscope just to be able to see and cut out the necessary tissue.

The published research on daylilies indicates that chromosome preparations for counting are not easy in this species.
Maurice
Last edited by admmad Jan 21, 2014 7:51 AM Icon for preview

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