Viewing post #542702 by chalyse

You are viewing a single post made by chalyse in the thread called Converting Tetraploids to Diploids - Is There a Process?.
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Jan 21, 2014 1:17 PM CST
Name: Tina
Where the desert meets the sea (Zone 9b)
Container Gardener Salvias Dog Lover Birds Enjoys or suffers hot summers Million Pollinator Garden Challenge
Garden Ideas: Level 2
So many steps forward, thank you @admmad. Thumbs up I had only found articles about the ubiquitous onion root tip squash method (as well as lily and orchid), and I guess it may be that non-household chemicals are needed in the process to view any plant chromosomes.

But, there is also that more coarse and inexact method outlined to distinguish dip from tet (no mention of trips...might they be sized by having length between dip and tet?) by measuring guard cells in the leaf:

http://www.hostalibrary.org/mi...

Any thoughts on whether this would work well enough to get a general idea about ploidy? If it were, I have seen some digital microscopes (100-800x) that have either measuring software or built-in reticles (at about 10 micron lengths) running anywhere from $30-100, with free shipping and a return option. At the lower-end price, and with no non-household chemicals needed, I might just be tempted to bite. Thoughts?
Do not seek to follow in the footsteps of old; seek what those of old sought. — Basho

Daylilies that thrive? click here! Thumbs up
Last edited by chalyse Jan 21, 2014 1:55 PM Icon for preview

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