Rick, a seed from one of his crosses tested 36-3-54. It is also interesting to note that Anastasia, commonly referred to as 3n, actually favors 4n at 17-14-60.
This is the first time this hybridizer has had samples tested. He says the test results raised more questions than they answered, so many of the theoretical and hypothetical questions I have at this point are the same questions he has. He plans to have more testing done this year. There are two people I know of who could probably answer some of them (Ms. Freeman, Dr. Griesbach), so as time permits, I'll compose my list of questions and ask them.
Fact: The sample was taken from a solid outer scale of a fresh dug bulb.
Question: What is the best location to take a sample? For the sake of uniformity, is there a standard procedure setting sampling location, time and age of the plant, etc.?
My Thoughts: While the test results greatly surprised me, I was not totally shocked. I was told some time ago that even in humans, our counts can differ slightly from organ to organ, especially in newborns and from one to another. We are not necessarily 100% 2n. I wouldn't be shocked to find somewhat differing results from scales, stems, leaves (even new and old), or buds. So, if I were to start a testing program, I would sample a center location mature leaf of the plant right at breeding time or point of pollination. I suppose the Dutch have such a standard procedure but it's most likely proprietary.
Question: Can tissue culture somehow alter counts (as opposed to the traditional or natural) ?
It's interesting to note that all 20 plus bulbs tested were obtained from Holland and were tissue cultured. Could this be why the Anastasia tested had counts favoring 4n?
Della, I copied your questions exactly and they will be added to the long list of questions the biologists at the university will be asked the next time testing is done. I think you may even have more ideas and questions you might think of; add them when they come to mind. I didn't have any trouble coming up with about a dozen theoreticals.
